Determination of amobarbital and secobarbital in plasma samples using micellar liquid chromatography
| Autor: | Rosa María Villanueva-Camañas, Yolanda Martín-Biosca, M.J. Medina-Hernández, Salvador Sagrado, C. Quiñones‐Torrelo |
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| Rok vydání: | 2000 |
| Předmět: |
Pharmacology
Detection limit Chromatography Plasma samples Chemistry Butabarbital Amobarbital Clinical Biochemistry Analytical chemistry General Medicine Secobarbital Biochemistry Analytical Chemistry Dilution Micellar liquid chromatography Drug Discovery medicine Uv detection Molecular Biology medicine.drug |
| Zdroj: | Biomedical Chromatography. 14:287-292 |
| ISSN: | 1099-0801 0269-3879 |
| DOI: | 10.1002/1099-0801(200008)14:5<287::aid-bmc1>3.0.co;2-c |
| Popis: | A new liquid chromatographic procedure for the determination of amobarbital and secobarbital in plasma samples is proposed. The method uses a Spherisorb octadecylsilane ODS-2 C18 analytical column, a guard column of similar characteristics and 0.04 M CTAB solution buffered at pH 7.5 containing 3% 1-propanol as micellar mobile phase. The UV detection was carried out at 250 nm. Butabarbital was used as internal standard. Plasma samples preparation only required adequate dilution with the mobile phase before injection into the chromatographic system. The limits of detection were 0.2 and 0.4 mg/L for amobarbital and secobarbital, respectively. The proposed method allows the determination of amobarbital and secobarbital in plasma at therapeutic levels. Copyright © 2000 John Wiley & Sons, Ltd. |
| Databáze: | OpenAIRE |
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