Control of column influence on the wide range pH gradient in ion-exchange chromatography

Autor:	K. Šlais, Z. Friedl
Rok vydání:	1992
Předmět:	Range (particle radiation) Sorbent Chromatography Chemistry Organic Chemistry Clinical Biochemistry Ion chromatography Analytical chemistry PH profile Biochemistry Analytical Chemistry Phase composition Phase (matter) Ph gradient Immobilized pH gradient
Zdroj:	Chromatographia. 33:231-236
ISSN:	1612-1112 0009-5893
DOI:	10.1007/bf02276188
Popis:	A method for optimization of the mobile phase composition in ion exchange chromatography with a wide range external pH gradient (approx. over 5 pH units) has been formulated. It is shown that programming of various wide range pH profiles which are not impaired by buffer interactions of the mobile phase with sorbent is possible. Utilization of the wide range external pH gradient in cation-exchange chromatography of bovine haemoglobin is also demonstrated.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::addafb005b13f4129e7c55d58cad1193 https://doi.org/10.1007/bf02276188 Zobrazit plný text záznamu Full text from SpringerLink