Comparison of early-antigen immunoperoxidase test in 18-h cultures and conventional virus isolation for the routine detection of cytomegalovirus in urine specimens

Autor:	Bruno Pozzetto, Odette G. Gaudin, F.C. Berthoux, C. Guérin, A. Ros
Rok vydání:	1990
Předmět:	Microbiology (medical) Pathology medicine.medical_specialty biology Immunoperoxidase Virus isolation Immunology Congenital cytomegalovirus infection Urine biology.organism_classification medicine.disease medicine.disease_cause Virology Virus Herpesviridae Antigen Betaherpesvirinae medicine
Zdroj:	Serodiagnosis and Immunotherapy in Infectious Disease. 4:101-107
ISSN:	0888-0786
DOI:	10.1016/0888-0786(90)90033-k
Popis:	Five hundred and thirty-one urine specimens were tested for the presence of cytomegalovirus (CMV) by conventional virus isolation (CVI) and early-antigen immunoperoxidase test (EAIPT) in 18-h cultures. After the exclusion of 44 uninterpretable results, concordant results were noted in 466 specimens out of 487 (95·7%) (421 negative (86·4%) and 45 positive (9·2%); five (1·0%) specimens were positive by CVI and negative by EAIPT and 16 (3·3%) gave the opposite pattern. As compared to CVI, EAIPT was 96·3% specific and 90·0% sensitive. When reviewing all the 531 specimens, 51 were positive by CVI and 74 by EAIPT (P 17 44 for EAIPT and 28 44 for CVI). Thus, EAIPT can be proposed as a more rapid and sensitive alternative to CVI for the detection of CMV in urine specimens.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::addaad5a8a02b8f40960d553a1246d71 https://doi.org/10.1016/0888-0786(90)90033-k Zobrazit plný text záznamu