Clinical value of serum microRNA-195 expression in invasive ductal carcinoma of the breast

Autor: Mohammed E. Abd El-latif, Eman S. Ahmed, Mohammed El-Shafae, Eman G. Behiry, Asmaa Adel El-Fallah
Rok vydání: 2020
Předmět:
Zdroj: Gene Reports. 19:100635
ISSN: 2452-0144
DOI: 10.1016/j.genrep.2020.100635
Popis: Background MicroRNAs (miR) are small molecules, with around 17–25 nucleotides in length. Dysregulation of miRNA expression can be used as a diagnostic biomarker of cancer because of their high stability in human serum. MiR-195 has been implicated as a diagnostic biomarker of breast cancer. Therefore, this study aimed to identify the expression levels of miR-195 and its diagnostic role in patients of breast cancer (BC). Methods The expression patterns of miR-195 in the serum was analysed from a total number of 100 female with invasive ductal carcinoma in addition to healthy control subjects. Isolation of total RNA was done, samples were measured using SYBR green-based real-time RT-PCR technology. Results Circulating levels of miR-195 were found to be significantly decreased in BC group compared to that of control group (p Conclusion Our data showed that serum miR-195 expressions were down-regulated in breast cancer females; furthermore, this downregulation was associated with higher specificity in diagnosis BC cases and so it could be used as a non-invasive diagnostic marker in BC.
Databáze: OpenAIRE
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