A SIMPLE AND SENSITIVE APCI-LC-MS METHOD FOR THE DETECTION OF THE ANTITUMOR AGENT, CARMUSTINE (BCNU) IN RAT PLASMA

Autor:	Russell G. Kerr, Abhijeet S. Kate, Amber C. Kohl
Rok vydání:	2010
Předmět:	Detection limit Carmustine Chromatography Chemistry Clinical Biochemistry Pharmaceutical Science Atmospheric-pressure chemical ionization Biochemistry Analytical Chemistry Adduct Hexane chemistry.chemical_compound Liquid chromatography–mass spectrometry Mass spectrum medicine Ion trap medicine.drug
Zdroj:	Journal of Liquid Chromatography & Related Technologies. 33:818-824
ISSN:	1520-572X 1082-6076
DOI:	10.1080/10826071003684331
Popis:	A simple and sensitive LC-MS method for the quantitative determination of the antitumor agent, N,N′-bis (2-chloroethyl)-N-nitrosourea (BCNU or carmustine), is described in this report. The method has been developed using an atmospheric pressure chemical ionization (APCI) probe on an ion trap mass spectrometer. A formate adduct ([M + 45]−) of BCNU was observed in the negative mode at 257.8 m/z and further confirmation for BCNU was obtained by observing the characteristic isotopic pattern in the mass spectrum, which indicated the compound contained two chlorine atoms. The extraction of BCNU from rat plasma with isopropyl ether:hexane (1:1) resulted in 81.3% of sample recovery. The limit of detection (LOD) calculated for the method was 1.25 ng (injected amount) using a standard solution of BCNU (S/N = 9:1). Optimum linearity and reproducibility data were obtained for BCNU; r2 = 0.9999 (0.2 µg/mL to 10.2 µg/mL) with precision of (RSD) ≤ 6.4% at two different concentrations and accuracy of 92.3 and 102.4%.
Databáze:	OpenAIRE
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