132: TGF-β polymorphisms, gender and age and their effect on acute cardiac rejection
| Autor: | Barry K. Rayburn, Christopher S. Coffey, Jose A. Tallaj, Robert C. Bourge, J.A. Barchue, Dawn Pekarek, Hernan E. Grenett, Raymond L. Benza, J.A. Kirklin |
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| Rok vydání: | 2007 |
| Předmět: |
Pulmonary and Respiratory Medicine
Transplantation medicine.drug_class business.industry Interleukin 1 receptor type II Age and gender Immunology Cohort Gene expression medicine Corticosteroid Surgery Cardiology and Cardiovascular Medicine Interleukin 1 receptor type I business Gene Transforming growth factor |
| Zdroj: | The Journal of Heart and Lung Transplantation. 26:S107 |
| ISSN: | 1053-2498 |
| DOI: | 10.1016/j.healun.2006.11.148 |
| Popis: | determinant of this discrimination was differential expression of 2 corticosteroid responsive genes, IL1R2 and FLT3. We have expanded these observations by analyzing 55 genes regulated by corticosteroids or immune genes that may influence corticosteroid responsiveness. Methods and Materials: A cohort of 73 patients (30-180 days post transplant; no current ACR) underwent RT-PCR gene expression analysis for 55 genes as above. Of these, 28 patients suffered 2R rejection in the next 12 weeks while 45 remained free of significant rejection. Results: 16 of 55 genes (including 13 not previously tested) significantly discriminated patients with future rejection from those without (p 0.05). Expression of 7 steroid responsive genes was significantly decreased in those with future rejection, whereas 4 T-cell activation genes were increased. The Il-1 pathway showed particular significance as IL1R1 and IL1R2 were decreased with future rejection, whereas IL1-beta was increased (all 0.05). Conclusions: These results suggest that RT-PCR analysis of corticosteroid regulated and alloimmune and inflammatory pathway genes allows for refined prediction of future occurrence of cardiac allograft rejection. These observations will serve to develop metrics to monitor corticosteroid adequacy and may allow preemptive modulation to prevent future rejection. |
| Databáze: | OpenAIRE |
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