Initiation of non-physiological division and manipulation of developmental pathway in cultured microspores ofMedicago sp

Autor: Andrew E. Moore, Gregory J. Tanner, Sergio Arcioni, Philip J. Larkin, M. Piccirilli
Rok vydání: 1990
Předmět:
Zdroj: Protoplasma. 158:165-175
ISSN: 1615-6102
0033-183X
DOI: 10.1007/bf01323129
Popis: Lucerne (alfalfa —Medicago sativa) pollen, cultured at the late unicellular stage, followed one of two developmental pathways: (1) A pathway involving symmetric mitosis which produces pollen containing two vegetative (2 V) or two generative (2 G) pollen. This morphology was only observed in culture, and pollen which followed this developmental path is defined as non-physiological. Occasionally the formation of multi-nucleate pollen grains containing from 4–9 cells were observed. Sustained divisions were not observed. (2) The production of bicellular (V+G) pollen followed by tricellular (V+2G) pollen. Since these types of grains are encountered during development in vivo, pollen following this developmental pathway is defined as physiological. The proportion of pollen that divided was enhanced by a cold treatment at 4°C for one week, prior to culture. The ratio of non-physiological (i.e., 2V or 2G) to physiological pollen (G+V or 2G+V) was found to be affected by the nature of the osmoticum in the medium. Media containing maltose or melibiose gave higher proportions of non-physiological pollen than media containing glucose or sucrose.
Databáze: OpenAIRE
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