Comparison of metal oxide catalysts for pyrolytic MOLI–MS bacterial identification

Autor:	Nicholas R. Saichek, Peter de B. Harrington, Kent J. Voorhees, Kirk R. Jensen, Christopher Cox
Rok vydání:	2015
Předmět:	chemistry.chemical_classification Cerium oxide Oxide Analytical chemistry Fatty acid Analytical Chemistry Catalysis Metal Chemometrics chemistry.chemical_compound Fuel Technology chemistry Component analysis visual_art visual_art.visual_art_medium Calcium oxide Nuclear chemistry
Zdroj:	Journal of Analytical and Applied Pyrolysis. 113:78-83
ISSN:	0165-2370
DOI:	10.1016/j.jaap.2014.10.016
Popis:	Six metal oxide catalysts were investigated for effective cleavage and laser ionization of bacterial cell wall phospholipid extracts. Calcium oxide has been utilized in the past for this application, but suffered from degradation of catalytic activity. Following evaluation of the six catalysts, CeO2 was found to be the most stable over time and produced fatty acid profiles of bacterial pathogens similar to freshly activated CaO. A suite of ten bacteria previously studied using CaO catalysis provided cross validation results of analysis of 97% correct correlation for negative-ion data. Cerium oxide catalysis studies yielded cross validation results of 100%. Using analysis of variance–principal component analysis (ANOVA–PCA), four sample sets collected with stored catalyst at 0, 8, 24, and 504 h showed no effect based on long-term catalyst degradation. Supervised learning by a fuzzy rule-building expert system (FuRES) that was validated with training and prediction set partitions independent of CeO2 age and unsupervised data analysis using a dendrogram of Euclidean distance confirmed that the CeO2 catalyst age had no effect on the fatty acid mass spectral profiles.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::ad7e1fcfe9011e81fee8e12d82a1d0cc https://doi.org/10.1016/j.jaap.2014.10.016 Zobrazit plný text záznamu Full Text from ScienceDirect