THE PURIFICATION OF MELANOMA ANTIGEN FROM HUMAN URINE

Autor:	Claire Bennett, K.B. Cooke
Rok vydání:	1978
Předmět:	Gel permeation chromatography Antiserum Proteases Electrophoresis Chromatography Affinity chromatography Biochemistry Chemistry Melanoma medicine Fractionation Urine medicine.disease
DOI:	10.1016/b978-0-08-022632-3.50034-9
Popis:	SUMMARY 1) Patients suffering from malignant melanoma or the associated benign condition of halo naevus excrete a specific melanoma associated protein in their urine. 2) Purification of this protein by gel chromatography, high voltage electrophoresis and salt fractionation gave low yields because of contaminating proteases in the urine. 3) Affinity chromatography of native urine on a column of rabbit anti-melanoma antiserum bound to AH-Sepharose 4B gave a single electrophoretic component in good yield. 4) 3M KCNS and 1 M propionic acid pH 2.5 were equally effective as desorbents. 5) Rechromatography on a dummy column resulted in significant loss of specific protein.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::ad77c9f504646e2824f1d3fb9b7956d9 https://doi.org/10.1016/b978-0-08-022632-3.50034-9 Zobrazit plný text záznamu