The Effects of Losartan in Preserving the Structural Integrity of Decellularized Small Diameter Vascular Allograft Conduit Implants In Vivo
| Autor: | Seong Who Kim, Seung Hyun Lee, Suk Jung Choo, Byoung Wook Lee |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Intimal hyperplasia Biomedical Engineering Medicine (miscellaneous) Bioengineering 030204 cardiovascular system & hematology Biomaterials 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Blood vessel prosthesis In vivo Medicine DAPI Decellularization business.industry General Medicine medicine.disease 030104 developmental biology Losartan medicine.anatomical_structure chemistry Immunohistochemistry business Elastic fiber Biomedical engineering medicine.drug |
| Zdroj: | Artificial Organs. 41:98-106 |
| ISSN: | 0160-564X |
| DOI: | 10.1111/aor.12686 |
| Popis: | Decellularization is a proposed method of preparing nonautologous biological arterial vascular scaffolding; however, the fate of the supporting medial elastic fiber, which is important in preserving the vascular structural integrity, is uncertain. The influence of losartan on preserving the medial elastic fiber integrity in decellularized small diameter vascular conduits (SDVC) was investigated. Decellularized infrarenal abdominal aortic allografts were implanted in Sprague-Dawley rats treated either with (study rats, n = 6) or without oral losartan (control rats, n = 6) and graded 8 weeks later according to a remodeling scoring system (1-mild, 2-moderate, 3-severe) which we devised based on the intimal hyperplasia degree, morphologic changes, and elastic fiber fragmentation of the conduits. DAPI immunohistochemistry analysis was performed in 47 (25 decellularization only and 22 losartan treatment) cross-sectional slide specimens. The losartan versus decellularization only SDVC showed a significantly lower medial elastic fragmentation score (1.32 vs. 2.24, P < 0.001), superior medial layer preservation, and relatively more normal appearing intimal cellular morphology. The results suggested rats receiving decellularized SDVCs treated with losartan may yield superior medial layer elastic fiber preservation. |
| Databáze: | OpenAIRE |
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