Thin-layer chromatography: Direct bioautography as a method of examination of antimicrobial activity of selected Potentilla species
| Autor: | W. Zieliński, Barbara Majer-Dziedzic, Grzegorz Jóźwiak, Wioleta Jesionek, Monika Waksmundzka-Hajnos |
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| Rok vydání: | 2016 |
| Předmět: |
Biological studies
Chromatography biology 010405 organic chemistry Chemistry 010401 analytical chemistry Clinical Biochemistry Pharmaceutical Science Antimicrobial biology.organism_classification 01 natural sciences Biochemistry Thin-layer chromatography 0104 chemical sciences Analytical Chemistry Column chromatography Potentilla erecta Potentilla |
| Zdroj: | Journal of Liquid Chromatography & Related Technologies. 39:281-285 |
| ISSN: | 1520-572X 1082-6076 |
| Popis: | Simple method for preparing biologically active material from plant extract may consist of four steps: obtaining an extract from selected plant, analytical chromatography with biological detection and transition to preparative scale, preparative isolation of biologically identified bands/groups of bands from chromatogram, rechromatography of isolated fractions. The next step of proceeding may be a quality analysis of separated material by, e.g., thin-layer chromatography (TLC)-MS Interface or biological studies after isolation. Extracts obtained from four Potentilla species: P. erecta, P. collina, P. megalantha, and P. crantzi were separated on silica plates, developed with the nonaqueous eluent. Immersion—direct bioautography was chosen as a method of detection of bands’ activity for their selection for isolated bands. Antibacterial/bacteriostatic properties of marked areas was the preliminary criterion of choice the samples to the further examinations. Preparative thin layer chromatography allow... |
| Databáze: | OpenAIRE |
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