Nuclear SRP9/SRP14 heterodimer transcriptionally regulates 7SL and BC200 RNA expression
| Autor: | Daniel Gussakovsky, Evan P Booy, Mira JF Brown, Sean A McKenna |
|---|---|
| Rok vydání: | 2023 |
| Předmět: | |
| Zdroj: | RNA. :rna.079649.123 |
| ISSN: | 1469-9001 1355-8382 |
| Popis: | The SRP9/SRP14 heterodimer is a central component of signal recognition particle (SRP) RNA (7SL) processing and Alu retrotransposition. In this study, we sought to establish the role of nuclear SRP9/SRP14 in the transcriptional regulation of 7SL and BC200 RNA. 7SL and BC200 RNA steady state levels, rate of decay, and transcriptional activity were evaluated under SRP9/SRP14 knockdown conditions. Immunofluorescent imaging, and subcellular fractionation of MCF-7 cells, revealed a distinct nuclear localization for SRP9/SRP14. The relationship between this localization and transcriptional activity at 7SL and BC200 genes was also examined. These findings demonstrate a novel nuclear function of SRP9/SRP14 establishing that this heterodimer transcriptionally regulates 7SL and BC200 RNA expression. We describe a model in which SRP9/SRP14 co-transcriptionally regulate 7SL and BC200 RNA expression. Our model is also a plausible pathway for regulating Alu RNA transcription and is consistent with the hypothesized roles of SRP9/SRP14 transporting 7SL RNA into the nucleolus for post-transcriptional processing, and trafficking of Alu RNA for retrotransposition. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|