Solids retention time, influent antibiotic concentrations, and temperature as selective pressures for antibiotic resistance in activated sludge systems
| Autor: | Victoria Obergh, Majid Neyestani, Oscar Quiñones, Daniel Gerrity, Channah Rock, Eric R.V. Dickenson, Jean E.T. McLain |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Environmental Engineering
Tetracycline medicine.drug_class Sulfamethoxazole 0208 environmental biotechnology Antibiotics 02 engineering and technology 010501 environmental sciences Biology 01 natural sciences Trimethoprim 020801 environmental engineering Microbiology Minimum inhibitory concentration Antibiotic resistance Ampicillin Trimethoprim Resistance medicine Food science 0105 earth and related environmental sciences Water Science and Technology medicine.drug |
| Zdroj: | Environmental Science: Water Research & Technology. 3:883-896 |
| ISSN: | 2053-1419 2053-1400 |
| DOI: | 10.1039/c7ew00171a |
| Popis: | This study evaluated the occurrence and potential proliferation of antibiotic resistance during biological wastewater treatment as a function of solids retention time (SRT), influent antibiotic concentrations, and temperature. Two phases of experiments were performed in laboratory-scale sequencing batch reactors (SBRs) fed with primary effluent from a full-scale wastewater treatment plant. Phase 1 evaluated SRTs of 2, 7, and 20 days with ambient antibiotic concentrations, and phase 2 evaluated a constant SRT of 7 days with influent antibiotic concentrations of 1×, 10×, and 100× relative to ambient levels. Ampicillin, sulfamethoxazole/trimethoprim, tetracycline, and vancomycin resistance were evaluated among Gram positive cocci (Staphylococcus and Streptococcus) using spread plate and minimum inhibitory concentration (MIC) assays. The laboratory-scale data demonstrated that biological treatment, in addition to longer SRTs, higher influent antibiotic concentrations, and higher temperatures, often resulted in greater relative prevalence of antibiotic resistance (up to 35% of the target population), and antibiotic resistant isolates were generally resistant to antibiotic concentrations 32 times higher than their baseline MICs. Some of these relationships were antibiotic-specific, with SRT having no significant impact on tetracycline resistance, influent antibiotic concentration having no significant impact on sulfamethoxazole/trimethoprim resistance, and temperature having no significant impact on vancomycin resistance. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|