Simultaneous monitoring of the enantiomeric purities of both substrate and product in the enzymatic resolution of (R,S)-2-acetoxy-3-bromopropylpara-toluenesulfonate by HPLC chiral column chromatography

Autor:	G. M. Chen, Grace F.S. Yin, C. M. Kiang, Y. G. Chang
Rok vydání:	1995
Předmět:	Pharmacology chemistry.chemical_classification Chromatography biology Resolution (mass spectrometry) Organic Chemistry Substrate (chemistry) High-performance liquid chromatography Catalysis Analytical Chemistry Chiral column chromatography Enzyme chemistry Drug Discovery biology.protein Lipase Enantiomer Enantiomeric excess Spectroscopy
Zdroj:	Chirality. 7:20-22
ISSN:	1520-636X 0899-0042
DOI:	10.1002/chir.530070104
Popis:	The enantiomeric purities of both substrate, 2-acetoxy-3-bromopropyl para-toluenesulfonate (I), and product, 2-hydroxy-3-bromopropyl p-toluenesulfonate (II) were examined in one analysis. The enzymatic resolution was conducted by Amano lipase AK and the enantiomeric excess as well as the conversion rate were monitored by HPLC analysis utilizing a Chiralcel OD column. After 7 h of reaction, HPLC results indicated that the enantiomeric purities of both substrate (I) and product (II) were greater than 95% and the conversion rate was around 55%. © 1995 Wiley-Liss, Inc.
Databáze:	OpenAIRE
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