Sub-5-minute Detection of SARS-CoV-2 RNA using a Reverse Transcriptase-Free Exponential Amplification Reaction, RTF-EXPAR

Autor:	Matthew R. Hicks, Jean-Louis H. A. Duprey, Marium Rana, Andrew Bosworth, Craig D. Southern, Jake G. Carter, Andrew D Beggs, Timothy R. Dafforn, Lorea Orueta Iturbe, James H. R. Tucker, Ian R. Carter
Rok vydání:	2021
Předmět:	2019-20 coronavirus outbreak chemistry.chemical_compound Coronavirus disease 2019 (COVID-19) chemistry Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA Single step Molecular biology DNA Virus Reverse transcriptase
DOI:	10.1101/2020.12.31.20248236
Popis:	We report a rapid isothermal method for detecting SARS-CoV-2, the virus responsible for COVID-19. The procedure uses a novel reverse transcriptase-free (RTF) approach for converting RNA into DNA, which triggers a rapid amplification using the Exponential Amplification Reaction (EXPAR). Deploying the RNA-to-DNA conversion and amplification stages of the RTF-EXPAR assay in a single step results in the detection of a sample of patient SARS-CoV-2 RNA in under 5 minutes.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::ad0a658cf0f568b24a45aa12d4a03659 https://doi.org/10.1101/2020.12.31.20248236 Zobrazit plný text záznamu