Technical Report: Assessment of Viability and Osteogenic Ability of Human Mesenchymal Stem Cells After Being Stored in Suspension for Clinical Transplantation

Autor:	Yoichi Kato, Kaori Muraki, Hajime Ohgushi, Hiroko Machida, Noriko Kotobuki, Yoshinori Takakura, Motohiro Hirose
Rok vydání:	2006
Předmět:	medicine.medical_treatment Mesenchymal stem cell General Engineering Biology Matrix (biology) In vitro Cell biology Transplantation In vivo medicine High alkaline phosphatase Saline Multipotential stem cell Biomedical engineering
Zdroj:	Tissue Engineering. 12:1711-1719
ISSN:	1557-8690 1076-3279
DOI:	10.1089/ten.2006.12.1711
Popis:	Human mesenchymal stem cells (MSCs) were suspended in phosphate-buffered saline (PBS) and stored up to 24 h at 4 degrees C, 24 degrees C, and 37 degrees C. More than 80% viability was maintained at any temperature for at least 1 h, then gradually decreased over time. After 24 h, the viabilities at 4 degrees C, 24 degrees C, and 37 degrees C were about 81%, 70%, and 62%, respectively. The MSCs suspended/stored in PBS at 4 degrees C for 24 h also exhibited in vitro osteogenic differentiation capability as evidenced by mineralized matrix formation as well as high alkaline phosphatase activity when cultured in an osteogenic medium. Furthermore, in vivo implantation experiments using the MSCs also demonstrated new bone formation. Because MSCs are known to possess multipotential stem cell characteristics, these data indicate that human MSCs stored in PBS at 4 degrees C could be delivered to distant medical facilities for the purpose of hard tissue and other types of tissue regeneration therapy.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::ad093834e5151b0a7467367c897b54b6 https://doi.org/10.1089/ten.2006.12.1711 Zobrazit plný text záznamu