Endogenous Nitric Oxide Suppresses Rat Myometrial Connexin 43 Gap Junction Protein Expression during Pregnancy1
| Autor: | Andrea Westerhausen-Larson, James M. Roberts, Stephen M. Sladek |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
medicine.medical_specialty
biology Myometrium Uterus Connexin Cell Biology General Medicine Endothelial NOS Oxytocin receptor Nitric oxide Nitric oxide synthase chemistry.chemical_compound medicine.anatomical_structure Endocrinology Reproductive Medicine chemistry Internal medicine biology.protein medicine Gap-43 protein |
| Zdroj: | Biology of Reproduction. 61:8-13 |
| ISSN: | 1529-7268 0006-3363 |
| DOI: | 10.1095/biolreprod61.1.8 |
| Popis: | Nitric oxide (NO) synthase (NOS) is active in the gravid uterus, and its activity decreases prior to the onset of parturition. We tested the hypothesis that NO helps maintain uterine quiescence by suppressing the expression of genes necessary for parturition. Pregnant rats (18 days gestation) were treated with inducible NOS (iNOS) inhibitor N-iminoethyl-L-lysine (NIL) or endothelial NOS inhibitor nitro-L-arginine methyl ester (L-NAME); 24 h later, uteri were analyzed for myometrial connexin 43 (Cx43) protein by immunoblotting and mRNA by Northern analysis. Myometrial oxytocin receptors (OTR) were measured by radioligand binding, and decidual prostaglandin H synthase (PGHS) protein by immunoblotting. Uterine NOS blockade was verified by NOS activity assay. We found that NIL, but not L-NAME, significantly increased myometrial Cx43 protein to parturitional levels with treatment at 19 but not 17 days gestation. Steady state mRNA concentrations were not changed at 24 h. NOS inhibition did not increase the concentrations of OTR, or PGHS protein, nor did it decrease maternal serum progesterone. We conclude that endogenous uterine NO from iNOS suppresses myometrial Cx43 gap junction protein expression during rat pregnancy. Although the exact mechanism is unknown, an increase of uterine wall stretch due to inhibition of relaxation could account for increased Cx43 gene transcription. |
| Databáze: | OpenAIRE |
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