MORPHOLOGIC APPEARANCE AND CYTOCHEMICAL PROPERTIES OF MALIGNANT CELLS FOLLOWING RAPID DEHYDRATION
| Autor: | Ezio Merler, Henry C. Orr, Morris Belkin, Walter G. Hardy |
|---|---|
| Rok vydání: | 1962 |
| Předmět: | |
| Zdroj: | Journal of Histochemistry & Cytochemistry. 10:495-501 |
| ISSN: | 1551-5044 0022-1554 |
| DOI: | 10.1177/10.4.495 |
| Popis: | Cells of strains HeLa, D164, D189, D227 and P388 were grown on coverslips. The first four strains are of human cell origin; P388 is derived from a mouse leukemia. These coverslips were placed over phosphorus pentoxide in a desiccator which was rapidly exhausted, and permitted to remain so for an hour. Such dehydrated cells, examined with transmitted light and the phase contrast microscope, revealed considerable intact structural detail. The cell surface, nuclear membrane and cytoplasmic granules appeared well preserved. Adding a little distilled water to the dehydrated cells produced an improvement in visibility of many cellular components so that now such "restored" cells under transmitted light and phase contrast microscopy strikingly resembled living cells. When cytochemical tests were made for certain intracellular constituents, fat stained strongly with oil red O as did polysaccharide by the periodic acid-Schiff reaction. Giemsa also stained the cells well. Poor staining was obtained with hematoxylin and cosin, and with fast green, indicating adverse protein changes. In dehydrated cells, acid and alkaline phosphatase showed considerable activity, whereas cytochrome oxidase and succinic dehydrogenase appeared completely inactivated. The advantages of dehydration as a fixing procedure are (1) simplicity, (2) rapidity, (3) avoidance of damage due to chemical fixation, and (4) it permits storage for an indefinite period prior to further treatment. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|