Detection of Bartonella spp. DNA in Clinical Specimens Using an Internally Controlled Real-Time PCR Assay
| Autor: | John W. A. Rossen, Anneke M C Bergmans |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Bartonella
Groel gene Bartonella henselae Cat-scratch disease Biology bacterial infections and mycoses medicine.disease biology.organism_classification Virology Microbiology chemistry.chemical_compound Real-time polymerase chain reaction chemistry hemic and lymphatic diseases TaqMan medicine bacteria Endocarditis DNA |
| Zdroj: | PCR Detection of Microbial Pathogens ISBN: 9781603273527 |
| DOI: | 10.1007/978-1-60327-353-4_14 |
| Popis: | Bartonella henselae is the causative agent of cat-scratch disease (CSD), usually presenting itself as a -self-limiting lymphadenopathy. In this chapter an internally controlled Taqman probe-based real-time PCR targeting the groEL gene of Bartonella spp. is described. This assay allows for the rapid, sensitive, and simple detection of Bartonella spp. in samples from CSD or endocarditis suspects, and it is suitable for implementation in the diagnostic microbiology laboratory. |
| Databáze: | OpenAIRE |
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