| Autor: | Y Nagasaka, Tomoko Emoto, K Nakamura, Y Suminami, H Kato, F Numa |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Pharmacology
Cancer Research Chymotrypsin biology Biochemistry (medical) Clinical Biochemistry Pharmaceutical Science Alpha (ethology) Cancer Cell Biology medicine.disease Trypsin Molecular biology Alpha 1-antichymotrypsin Apoptosis Hepatocellular carcinoma biology.protein medicine DNA fragmentation medicine.drug |
| Zdroj: | Apoptosis. 3:155-160 |
| ISSN: | 1360-8185 |
| DOI: | 10.1023/a:1009694621397 |
| Popis: | Increased serum levels of alpha1-antichymotrypsin (alpha 1ACT) are observed in some cancer patients, especially those with hepatocellular carcinoma. A possible role of alpha 1ACT in tumour growth has been suggested, but this remains uncertain. We have demonstrated that alpha 1ACT inhibited chymotrypsin-induced apoptosis in rat hepatoma H4 cells. Even low concentrations of chymotrypsin (but not trypsin) induce apoptosis in H4 cells with a minimum effective concentration of 2.4 x 10(-2) units/ml (0.5 microg/ml), and this apoptosis was inhibited by alpha 1ACT in a concentration-dependent manner. Furthermore, the concentrations of alpha 1ACT required to inhibit the apoptosis were lower than normal serum levels. These results may indicate that alpha 1ACT plays a role in the apoptosis of rat hepatoma cells. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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