On the accuracy of protein determination in large biological samples by prompt gamma neutron activation analysis

Autor:	P. Papadopoulou, John Kalef-Ezra, E. Yannakopoulou, Ion E. Stamatelatos, K. Kasviki
Rok vydání:	2007
Předmět:	Nuclear and High Energy Physics Chemistry Small animal Radiochemistry Radiation dose Prompt gamma neutron activation analysis chemistry.chemical_element Meat sample Whole body Instrumentation Kjeldahl method Nitrogen Total protein
Zdroj:	Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms. 263:132-135
ISSN:	0168-583X
DOI:	10.1016/j.nimb.2007.04.073
Popis:	A prompt gamma neutron activation analysis (PGNAA) facility has been developed for the determination of nitrogen and thus total protein in large volume biological samples or the whole body of small animals. In the present work, the accuracy of nitrogen determination by PGNAA in phantoms of known composition as well as in four raw ground meat samples of about 1 kg mass was examined. Dumas combustion and Kjeldahl techniques were also used for the assessment of nitrogen concentration in the meat samples. No statistically significant differences were found between the concentrations assessed by the three techniques. The results of this work demonstrate the applicability of PGNAA for the assessment of total protein in biological samples of 0.25–1.5 kg mass, such as a meat sample or the body of small animal even in vivo with an equivalent radiation dose of about 40 mSv.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::acdaacaad333428637be8e5458fdc879 https://doi.org/10.1016/j.nimb.2007.04.073 Zobrazit plný text záznamu Full Text from ScienceDirect