| Popis: |
In order to search for new α-amylase inhibitors from Chinese Yam (Dioscorea opposita Thunb.), the extracts of alkali-soluble crude polysaccharides (ACP), water-soluble crude polysaccharides (WCP), total saponins, and flavones were isolated, and their inhibitory effects on α-amylase were evaluated. The experimental results show that the saponins and flavones had a marked inhibition effect on α-amylase, however, the ACP and WCP exhibited weakly inhibitory activities. The kinetic analysis indicates that the inhibition type of these extracts on α-amylase was competitive, and the enzyme-substrate apparent dissociation constant (Km’) of acarbose (the control), WCP, saponins, and flavones is 118.86, 32.46, 79.23, 49.51 mg/ml, respectively. |
