| Popis: |
Each of 14 commercial insulin preparations was assayed for insulin activity using three biological assay methods. In Assay I, 32 rabbits were employed using the "twin cross-over" design. Per cent reductions of blood sugar calculated from blood-sugar levels determined before and one and one-half, three, and five hours after the subcutaneous injection of insulin were used in estimating potency. In Assay II, 16 rabbits were used in a 4 × 4 Latin square design. Blood-sugar levels determined 50 min. after the intravenous injection of insulin were used in estimating potency. In Assay III, 288 mice were employed in a two-level quantal response design. The proportions of animals convulsing in groups of 36 mice after the subcutaneous injection of insulin were used in measuring potency. The agreement between results obtained by the three methods was found to be satisfactory. The average standard error for the estimate of potency was 13% for Assay I; 10% for Assay II; and 9% for Assay III. |
