Autor: |
Fasuba Ilesanmi Kayode, Adu Isaac Adekola, Oluwatoyin Alice Tella, Olaiya Oluranti Esther, Akawa Ayodeji Benjamin, Akawa Oluwole Bidemi |
Rok vydání: |
2021 |
Předmět: |
|
Zdroj: |
International Journal of Pharmacy and Chemistry. 7:101 |
ISSN: |
2575-5730 |
DOI: |
10.11648/j.ijpc.20210705.13 |
Popis: |
When oxidation by free radicals exceeds antioxidant systems in the body, oxidative stress usually results. This is common in the underlying processes leading to aging, infertility and of a range of non-communicable diseases including cancers, cardiovascular diseases, neurodegenerative diseases and rheumatoid arthritis. There exist measures to curb oxidative stress, one of which is to take in exogenous antioxidants in form of supplements and in diet. Studies have shown that certain plants have antioxidant and free radical scavenging activity. The aim of this study is to evaluate the free radical scavenging activity (FRSA) of the crude extract of Platostoma africanum P. Beauv. (Lamiaceae). The crude extract was partitioned using N-hexane, Di-ethylether, Ethyl acetate and N-butanol in that order. TLC phytochemical screening as well as DPPH bioautographic analysis was done for each fraction as well as the residual aqueous fraction in suitable mobile phase system in order to evaluate their FRSA and secondary metabolites. A DPPH assay was then carried out using ultraviolet spectroscopy to quantify the activity. The fraction with the lowest IC50 and highest activity was further separated into fractions by column chromatography. DPPH bioautography and phytochemical screening were done on the resulting fractions. The crude as well as the five fractions had FRSA in varying degrees. Ethyl acetate fraction had the lowest IC50 (0.42mg/mL) and the highest activity. The decreasing order of activity was ethyl acetate>diethyl ether>n-butanol>crude>aqueous>n-hexane. Among the fractions obtained from column chromatography, fraction D was the most active. Terpenoids and flavonoids were the main secondary metabolites observed in the plant and were responsible for the FRSA. Platostoma africanum P. Beauv. has antioxidant activity which is mostly due to its terpenoid and flavonoid content. |
Databáze: |
OpenAIRE |
Externí odkaz: |
|