Reusable System for Phenol Detection in an Aqueous Medium Based on Nanodiamonds and Extracellular Oxidase from Basidiomycete Neonothopanus nambi

Autor:	Vladimir S. Bondar, O. A. Mogilnaya, E. D. Posokhina, N. O. Ronzhin
Rok vydání:	2021
Předmět:	Aqueous solution Immobilized enzyme Chemistry Biophysics General Chemistry General Medicine Biochemistry Catalysis chemistry.chemical_compound Adsorption Yield (chemistry) Phenol Hydrogen peroxide Neonothopanus nambi Nuclear chemistry
Zdroj:	Doklady Biochemistry and Biophysics. 499:220-224
ISSN:	1608-3091 1607-6729
DOI:	10.1134/s1607672921040141
Popis:	A reusable system for phenol determination in an aqueous medium was obtained by adsorption of extracellular oxidase from fungus Neonothopanus nambi onto modified nanodiamonds (MND) synthesized by detonation. It was found that the enzyme strongly binds to MND and exhibits catalytic activity in the reaction of co-oxidation of phenol with 4-aminoantipyrine without the addition of hydrogen peroxide. In the presence of the MND-oxidase complex, a significantly (by an order of magnitude) higher yield of the reaction product is recorded as compared to the yield in the presence of a free enzyme; the mechanism of the revealed effect is discussed. Model experiments have demonstrated the multiple use of the MND-oxidase complex for testing phenol in aqueous samples. The immobilized enzyme exhibits functional activity during long-term (2 months) storage of the MND-oxidase complex at 4°C. The data obtained create the prerequisites for using the created system in environmental monitoring of water pollution with phenol.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::ac96591192afeab8dab82568cd2c546b https://doi.org/10.1134/s1607672921040141 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
Nepřihlášeným uživatelům se plný text nezobrazuje	K zobrazení výsledku je třeba se přihlásit.