Selective removal of IgG from the urine of patients with proteinuria using a polymer coated core–shell magnetic nanoparticle
| Autor: | Huifang Du, Yuan Xuesheng, Kai Hu, Shusheng Zhang, Zhifen Deng, Hang Yuan, Liangliang Bi, Shengnan Zhao, Yanjie Huang, Yanlong Chen |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
chemistry.chemical_classification
Thermogravimetric analysis Materials science General Chemical Engineering Analytical chemistry Nanoparticle Isothermal titration calorimetry 02 engineering and technology General Chemistry Polymer 010402 general chemistry 021001 nanoscience & nanotechnology 01 natural sciences 0104 chemical sciences Adsorption chemistry Polymerization Selective adsorption Magnetic nanoparticles 0210 nano-technology Nuclear chemistry |
| Zdroj: | RSC Advances. 6:107732-107738 |
| ISSN: | 2046-2069 |
| DOI: | 10.1039/c6ra24560a |
| Popis: | Selective removal of highly abundant proteins prior to analysis is an important issue in proteomics. In this study, a core–shell structured magnetic nanocomposite of Fe3O4@SiO2@MAsp-HEMA was found to be an effective and selective adsorbent to remove highly abundant IgG from the urine of patients with proteinuria. This novel magnetic adsorbent was prepared by coating a poly(N-methacryloyl-L-aspartic acid-hydroxyethyl methacrylate) shell on Fe3O4@SiO2 nanoparticles via a coupling agent of KH-570 using the mini-emulsion polymerization method. The as-synthesized nanoparticle was characterized by X-ray diffraction, elemental analysis, thermal gravimetric analysis, infrared spectroscopy, magnetic hysteresis loop curves, scanning electronic microscopy and transmission electron microscopy. A kinetic adsorption experiment was performed to investigate the speed of adsorption and the time to reach the adsorption balance. 1D SDS-PAGE analysis and capillary electrophoresis were used to validate its selective adsorption ability. The resulting Fe3O4@SiO2@MAsp-HEMA showed a uniform spherical shape at nanoscale dimensions of about 200–300 nm, removing efficiency above 80%, and maximum adsorption capacity of 18.08 μg mg−1 at 15 ± 0.5 °C. The binding between IgG and the ligand was confirmed by isothermal titration calorimetry. The hydrogen bonding force was demonstrated as one of the major interactions between the functional groups of the as-synthesized magnetic nanoparticles and IgG by a molecular docking experiment. |
| Databáze: | OpenAIRE |
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