Cytogenetic and immunological study of mammalian hybrid cell lines

Autor: L. K. Ernst, E.A. Gladyr, P. M. Klenovitskiy, N.A. Zinovieva, Ju. V. Konopelko, N. M. Grezina
Rok vydání: 2007
Předmět:
Zdroj: Cell and Tissue Biology. 1:133-142
ISSN: 1990-5203
1990-519X
Popis: Two hybrid cell lines, KS-RL-3 (hybrid between TK− sheep kidney cells and rabbit lymphocytes) and CR-KS TK− (hybrid between rabbit β-cells and TK− sheep kidney cells), were assayed cytogenetically. It was shown that these hybrid cell lines were characterized by the presence of both sheep and rabbit chromosomes, with a number and structure which varied depending on the cell type and the number of passages. In some cases aberrant chromosomes were identified. The modal chromosome number was 121–135 (40.7%) in the KS-RL-3 cell line and 106–120 (51.6%) in the CR-KS TK− cell line. CR-KS TK− and KS-RL-3 cells were identified in the testicles and ear lobes of experimental animals during periods of 7 to 28 days after cell inoculation. Partial immunological tolerance of the hybrid cell lines was suggested.
Databáze: OpenAIRE
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