HPV Testing in Liquid Cytology Specimens
| Autor: | Patrick J. Ringer, David R. Bolick, Frank L. Spangler, Grenith Zimmerman, M. Nasar Qureshi |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Pathology
medicine.medical_specialty education.field_of_study Histology business.industry Population virus diseases General Medicine In situ hybridization Gold standard (test) medicine.disease Pathology and Forensic Medicine law.invention Squamous intraepithelial lesion law Liquid-based cytology Cytology Medicine business education Polymerase chain reaction Chemiluminescence |
| Zdroj: | Acta Cytologica. 49:120-126 |
| ISSN: | 1938-2650 0001-5547 |
| Popis: | Objective To compare the analytic sensitivity and specificity of Hybrid Capture 2 (HC2) (Digene Corporation, Gaithersburg, Maryland, U.S.A.) with in situ hybridization (ISH) in detecting high-risk types of HPV (HR-HPV). Study design Performance characteristics of ISH and HC2 were compared in 99 consecutive cervical cytology samples diagnosed as low grade squamous intraepithelial lesion and processed by either the ThinPrep (Cytyc Corporation, Boxborough, Massachusetts, U.S.A.) or SurePath (TriPath Imaging Systems, Research Triangle Park, North Carolina, U.S.A.) method at 2 geographically different centers. Polymerase chain reaction (PCR) was used as the gold standard. Results Of the samples, 67% were positive for HR-HPV viral types by PCR. ISH had a sensitivity of 0.87 as compared to 0.95 (p = 0.11) for HC2. The specificity of ISH was significantly different from that of HC2 (0.57 vs. 0.13, respectively; p = 0.0004). The performance characteristics of ISH were not affected by the processing method or population tested. Conclusion The sensitivity of ISH is comparable to that of HC2, with significantly superior specificity, and is therefore an efficacious alternative to HC2 for triaging patients with abnormal cervical cytology results. |
| Databáze: | OpenAIRE |
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