Resistin-like molecules reduce influenza A virus morbidity in mice (VIR5P.1158)
| Autor: | Amali Samarasinghe, Yanyan Lin, Kim LeMessurier, Stacie Woolard, Jonathan McCullers |
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| Rok vydání: | 2015 |
| Předmět: | |
| Zdroj: | The Journal of Immunology. 194:148.26-148.26 |
| ISSN: | 1550-6606 0022-1767 |
| DOI: | 10.4049/jimmunol.194.supp.148.26 |
| Popis: | Resistin-like molecules (RELMs) are recently discovered cysteine rich secreted proteins abundant in a variety of organs including the lungs. RELMα has an inhibitory role in the lungs by downregulating inflammation, while RELMβ promotes inflammation. We noted a markedly greater expression of RELMs in the lungs of mice with acute asthma and influenza, which were protected against severe influenza morbidity and hypothesized that RELMα and RELMβ proteins enhance anti-viral defenses. Recombinant RELMα and RELMβ proteins were administered intranasally to C57BL/6 mice starting three days prior to viral infection and continued on a daily basis following viral infection. Mice were infected with 1000 TCID50/ml of A/CA/04/2009 pandemic influenza A virus. Animals were sacrificed on days 3, 5, 7, and 9 following viral infection for sample acquisition. Animals not treated with exogenous RELMs served as controls. Administration of RELM proteins led to a reduction in airway resistance after influenza virus infection. Inflammation in the airways increased in RELM-treated mice with macrophages and neutrophils taking precedence and a prominent increase in CD8+ T cells at late timepoints. A significant reduction in viral burden occurred in the lungs of RELM treated mice. The administration of RELM proteins directly impacted the host immune response against influenza A virus. Future studies will investigate the mechanisms by which RELMs mediate protection against influenza. |
| Databáze: | OpenAIRE |
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