| Popis: |
Lung cancer is one of the most common malignancies and the leading cause of cancer-related deaths worldwide. Elucidating the mechanism behind the development of lung cancer at a molecular level could reveal new biomarkers and clinical therapeutic targets. A growing body of evidence has shown an association between ion channel-related genes and the progression of various diseases, including cancer. However, the association is not well-understood. In this study, we identified ion channel-related genes differentially expressed between cancer and normal lung tissue cells. Data were extracted from GSE31552, GSE33532, and GSE103512 lung adenocarcinoma (LUAD) datasets, and the prognostic value of the differentially expressed genes between LUAD and normal lung tissue was evaluated using data in The Cancer Genome Atlas. Only sodium voltage-gated channel alpha subunit 7 (SCN7A) was found to be significantly correlated to prognosis. The expression of SCN7A in LUAD was assessed further by immunological analysis. We also constructed a competing endogenous RNA network of SCN7A. Further analyses revealed that the underexpression of SCN7A predicted a poor prognosis in LUAD, with a strong correlation between SCN7A expression and immune cell infiltration as well as immune checkpoint expression. We found that SCN7A is potentially regulated via the OTUD6B-AS1-miR-21-5p-SCN7A axis in LUAD cells and proved that SCN7A inhibits the proliferation and migration of lung cancer cells in vitro. Taken together, SCN7A, as an independent prognostic factor, is a promising diagnostic biomarker for LUAD, and the OTUD6B-AS1-miR-21-5p-SCN7A axis is a potential regulatory network of SCN7A expression. |
