Evaluation of 'quick and dirty' DNA extraction methods for marker-assisted selection in rice (Oryza sativa L.)

Autor:	Bertrand C. Y. Collard, David J. Mackill, A. Das, Parminder S. Virk
Rok vydání:	2007
Předmět:	Oryza sativa Chloroform Chromatography business.industry Extraction (chemistry) Plant Science Biology Marker-assisted selection Isoamyl alcohol DNA extraction Biotechnology chemistry.chemical_compound chemistry Sodium hydroxide Genetics Sodium dodecyl sulfate business Agronomy and Crop Science
Zdroj:	Plant Breeding. 126:47-50
ISSN:	1439-0523 0179-9541
DOI:	10.1111/j.1439-0523.2006.01272.x
Popis:	Six simple methods for extracting DNA from rice seedlings were evaluated for marker-assisted selection (MAS). The assessment of each method was based on PCR amplification of SSR markers, DNA yield and purity, time and cost. Based on these criteria, two methods were selected as being superior to other methods. The best two methods included the standard method developed at the International Rice Research Institute (IRRI), which utilizes a sodium dodecyl sulfate extraction buffer followed by chloroform/isoamyl alcohol extraction and a previously published method using sodium hydroxide and Tris. These two methods produced nearly identical PCR amplification results. The sodium hydroxide method is considerably simpler, quicker and cheaper than the standard IRRI method, and may be particularly useful for many applications of MAS or high-resolution mapping. This method was also adapted into an effective high throughput method utilizing 96-well plates emphasizing its versatility.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::ac499af7af2d9cc36f6900adc694211a https://doi.org/10.1111/j.1439-0523.2006.01272.x Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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