Biotransformation of ferulic acid to acetovanillone usingRhizopus oryzae

Autor:	U. S. Narayana Murty, Rajendra Prasad, K. Hara Kishore, K. Shiva Shanker, Sunil Misra, Sanjit Kanjilal
Rok vydání:	2007
Předmět:	Chromatography biology Vanillin Ethyl acetate Rhizopus oryzae biology.organism_classification Biochemistry Catalysis Ferulic acid Vanillyl alcohol chemistry.chemical_compound chemistry Vanillic acid Organic chemistry Guaiacol Biotechnology Coniferyl alcohol
Zdroj:	Biocatalysis and Biotransformation. 25:109-112
ISSN:	1029-2446 1024-2422
DOI:	10.1080/10242420601141721
Popis:	Microbial transformation of ferulic acid to acetovanillone was studied using growing cells of Rhizopus oryzae. Ferulic acid was added to the growing medium (0.5 g L−1) and incubated for 12 days. The progress of formation of metabolites was monitored by GC and GC-MS after extraction with ethyl acetate. The major metabolite was acetovanillone with minor metabolites formed, such as dihydroferulic acid, coniferyl alcohol and dihydroconiferyl alcohol. Traces of metabolites (≤1–3%), such as vanillin, vanillyl alcohol, vanillic acid and phenyl ethyl alcohol, were also produced. Formation of 4-vinyl guaiacol increased from day 1 (12.4%), reaching a maximum on day 4 (31.7%), and reducing to a minimum on day 12 (3.1%). The formation of acetovanillone increased only from day 2 onward, and reached a maximum (49.2%) on day 12. The optimum concentration of ferulic acid to be added into the medium was found to be only 0.5 g L−1, as any increase in concentration (0.75 and 1.0 g L−1) precipitated the precursor, resulting ...
Databáze:	OpenAIRE
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