Effect of growth rate on stability of a recombinant plasmid during continuous culture of Saccharomyces cerevisiae in non-selective medium
| Autor: | P. Caunt, P.F. Greenfield, A. Impoolsup |
|---|---|
| Rok vydání: | 1989 |
| Předmět: |
chemistry.chemical_classification
Strain (chemistry) biology Saccharomyces cerevisiae Bioengineering General Medicine Chemostat biology.organism_classification Applied Microbiology and Biotechnology Yeast Microbiology law.invention Plasmid Enzyme chemistry law Recombinant DNA Biophysics Growth rate Biotechnology |
| Zdroj: | Journal of Biotechnology. 10:171-180 |
| ISSN: | 0168-1656 |
| DOI: | 10.1016/0168-1656(89)90039-4 |
| Popis: | The stability of a 2μm based plasmid, pLG669-z, was measured in a Saccharomyces cerevisiae strain, grown continuously in a non-selective medium. The apparent stability of the plasmid was found to decrease with increasing growth rate. A model was developed to predict the contributions of segregational instability and growth rate differences to the apparent stability. An increase in segregational instability was the dominant factor in the increased rate of plasmid loss. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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