A dihydrofolate reductase gene fromCandida albicans:molecular cloning

Autor:	M. Franceschi, Mario Polsinelli, R. Lorenzetti, M. Denaro, Giorgio Mastromei, E. Skarmoutsou, Tiziana Irdani
Rok vydání:	1991
Předmět:	biology Dihydrofolate reductase activity Molecular cloning biology.organism_classification Microbiology Molecular biology Trimethoprim Corpus albicans Restriction map Plasmid Genetics Trimethoprim Resistance medicine Candida albicans Molecular Biology medicine.drug
Zdroj:	FEMS Microbiology Letters. 80:179-182
ISSN:	1574-6968 0378-1097
Popis:	The dihydrofolate reductase gene from Candida albicans has been cloned and partially characterized. A genomic bank from C. albicans strain 10127/5 was constructed in Escherichia coli and screened for trimethoprim resistance. A plasmid pMF1, carrying the resistance marker was isolated and characterized by restriction mapping and Southern blotting. Cells harbouring pMF1 were as sensitive as the parental cells to a wide spectrum of antibacterial agents, except for trimethoprim; the dihydrofolate reductase activity from these cells was trimethoprim resistant.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::ac39199cd4c8c7abe90cdbf7a6213f04 https://doi.org/10.1111/j.1574-6968.1991.tb04657.x Zobrazit plný text záznamu Plný text