Crystal Structure and Regiospecificity of Catechol O-Methyltransferase from Niastella koreensis
| Autor: | Kyung-Jin Kim, Seul Hoo Lee, Bongsang Kim |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
inorganic chemicals
0106 biological sciences chemistry.chemical_classification Conformational change Catechol Catechol-O-methyl transferase biology Stereochemistry 010401 analytical chemistry General Chemistry medicine.disease_cause 01 natural sciences Cofactor 0104 chemical sciences chemistry.chemical_compound Enzyme chemistry biology.protein medicine Binding site General Agricultural and Biological Sciences Niastella koreensis 010606 plant biology & botany Methyl group |
| Zdroj: | Journal of Agricultural and Food Chemistry. 69:2531-2538 |
| ISSN: | 1520-5118 0021-8561 |
| DOI: | 10.1021/acs.jafc.0c07621 |
| Popis: | Catechol O-methyltransferase (COMT) is an enzyme that transfers a methyl group to the catechol-derivative substrates using S-adenosyl-l-methionine (SAM) and Mg2+. We report the biochemical and structural analysis of COMT from Niastella koreensis (NkCOMT). NkCOMT showed the highest activity with Mg2+, although the enzyme also showed a significant level of activity with Cu2+ and Zn2+. NkCOMT structures complexed with SAH and Mg2+ elucidated how the enzyme stabilized the cosubstrate and the metal ion and revealed that the region near the SAM binding site undergoes conformational changes upon the binding of the cosubstrate and the metal ion. We also identified the catechol binding pocket of the enzyme and explained a broad substrate specificity of the bacterial enzyme and its ability to accommodate the catechol derivatives. In addition, we developed the NkCOMTE211R and NkCOMTE211K variants that showed both enhanced activities and regiospecificity for the production of the para-forms. Our study provides a structural basis for regiospecificity of NkCOMT, which is related with the conformational change upon binding of SAM and Mg2+. |
| Databáze: | OpenAIRE |
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