Determination of Tilmicosin Residues In Chicken, Cattle, Swine, and Sheep Tissues by Liquid Chromatography

Autor:	Robin S. Readnour, James M Turner, Carolyn M Stobba-Wiley, John W Moran, Jen P Chang, David T Elsbury
Rok vydání:	2000
Předmět:	Pharmacology Detection limit Chromatography Chemistry Extraction (chemistry) Relative standard deviation Tissue sample Analytical Chemistry Matrix (chemical analysis) chemistry.chemical_compound Chicken fat Environmental Chemistry Tilmicosin Agronomy and Crop Science Food Science
Zdroj:	Journal of AOAC INTERNATIONAL. 83:837-846
ISSN:	1944-7922 1060-3271
DOI:	10.1093/jaoac/83.4.837
Popis:	A method was developed and validated for determination and quantitation of tilmicosin residues in swine, cattle, and sheep edible tissues, as well as chicken fat, skin, and muscle over a concentration range of 0.025 μg/g–20 μg/g. For chicken kidney and liver, the method was validated over a range of 0.060 μg/g–20 μg/g. The tissue sample was extracted with methanol and a C18 cartridge was used for solid-phase extraction cleanup. A reversed phase gradient liquid chromatographic method with detection at 280 nm was used to separate the tilmicosin from matrix components in 30 min run time. The limit of quantitation (LOQ) of the method was 0.025 μg/g for all tested tissues except chicken kidney and liver, for which the LOQ was 0.06 μg/g. Average recoveries for tissue samples ranged from 73 to 98%. Relative standard deviation values ranged from 0.6 to 14.7%.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::abfd5d84a5582fde9c5a5ebf025b48e9 https://doi.org/10.1093/jaoac/83.4.837 Zobrazit plný text záznamu