Analysis of peptide uptake and location of root hair-promoting peptide accumulation in plant roots
| Autor: | Motoki Kubo, Rikiya Taniguchi, Yoshiki Matsumiya |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Pharmacology
chemistry.chemical_classification Plant roots digestive oral and skin physiology fungi Organic Chemistry food and beverages Peptide General Medicine Biology Root hair biology.organism_classification Biochemistry Bioactive peptide chemistry Structural Biology Drug Discovery Brassica rapa Molecular Medicine Water volume Solanum Molecular Biology |
| Zdroj: | Journal of Peptide Science. 18:177-182 |
| ISSN: | 1075-2617 |
| Popis: | Peptide uptake by plant roots from degraded soybean-meal products was analyzed in Brassica rapa and Solanum lycopersicum. B. rapa absorbed about 40% of the initial water volume, whereas peptide concentration was decreased by 75% after 24 h. Analysis by reversed-phase HPLC showed that number of peptides was absorbed by the roots during soaking in degraded soybean-meal products for 24 h. Carboxyfluorescein-labeled root hair-promoting peptide was synthesized, and its localization, movement, and accumulation in roots were investigated. The peptide appeared to be absorbed by root hairs and then moved to trichoblasts. Furthermore, the peptide was moved from trichoblasts to atrichoblasts after 24 h. The peptide was accumulated in epidermal cells, suggesting that the peptide may have a function in both trichoblasts and atrichoblasts. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text