Application of an enzyme encapsulated metal-organic framework composite for convenient sensing and degradation of methyl parathion
| Autor: | Akash Deep, Neha Bhardwaj, Ki-Hyun Kim, Ashok K. Paul, Sarita Dhaka, Sung-Eun Lee, Jyotsana Mehta, Siddavattam Dayananda |
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| Rok vydání: | 2019 |
| Předmět: |
Detection limit
chemistry.chemical_classification Chemistry Metabolite Composite number Metals and Alloys 02 engineering and technology 010402 general chemistry 021001 nanoscience & nanotechnology Condensed Matter Physics 01 natural sciences Combinatorial chemistry 0104 chemical sciences Surfaces Coatings and Films Electronic Optical and Magnetic Materials Catalysis Highly sensitive chemistry.chemical_compound Enzyme Materials Chemistry Parathion methyl Metal-organic framework Electrical and Electronic Engineering 0210 nano-technology Instrumentation |
| Zdroj: | Sensors and Actuators B: Chemical. 290:267-274 |
| ISSN: | 0925-4005 |
| DOI: | 10.1016/j.snb.2019.03.116 |
| Popis: | Terbium-BTC (BTC = 1,2,4-Benzenetricarboxylic acid) metal-organic framework (MOF) has been employed for in-situ encapsulation of hexahistidine-tagged organophosphate hydrolase (OPH6His) enzyme. The enzymatic activity and stability of OPH6His were found remarkably enhanced after its encapsulation within the MOF. The potential of this enzyme-MOF composite (OPH6His@Tb-BTC) has been explored as a dual platform to simultaneously detect organophosphate pesticides (OPPs) and catalyze their degradation. The OPH6His@Tb-BTC exhibited improved enzymatic activity (by approximately 30%) and reusability (at least five cycles). It is demonstrated to be a highly sensitive and simple optical bioprobe for the detection of an OPP candidate, methyl parathion with a low limit of detection (2.6 nM). A less toxic metabolite was formed through catalytic conversion of methyl parathion (i.e., upon its incubation with OPH6His@Tb-BTC), further supporting the promising role of this composite in the remediation of pesticide-contaminated environments. |
| Databáze: | OpenAIRE |
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