| Autor: |
Shai Fleishon, Ella Mendelson, Neta S. Zuckerman, Oran Erster, Michal Mandelboim, Virginia Levy, Yaniv Ali, Batya Mannasse, Roberto Azar, Michal Elul, Itay Bar-Or, Danit Sofer, Dana Bar-Ilan, Orna Mor, Areej Kabat, Hadar Asraf, Efrat Bucris |
| Rok vydání: |
2021 |
| Předmět: |
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| Popis: |
In this report, we describe the development of an RT-qPCR assay, termed Alpha Delta assay, which can detect SARS-COV-2 (SC-2) and distinguish between the Alpha (B.1.1.7) and Delta (B.1.617.2) variants. The Alpha- and Delta-specific reactions in the assay target mutations that are strongly linked to the target variant. The Alpha reaction targets the D3L substitution in N gene, and the Delta reaction targets the spike gene 156-158 mutations. Additionally, we developed a second Delta-specific assay, used as a confirmatory test for the Alpha Delta assay that targets the 119-120 deletion in the Orf8 gene. Both reactions have similar sensitivities of 15-25 copies per reaction, similar to the sensitivity of commercial SC-2 detection tests. The Alpha Delta assay and the Orf8-119del assay were successfully used to classify clinical samples that were subsequently analyzed by whole genome sequencing. Lastly, we show that the Alpha Delta and Orf8-119del assays correctly identified the presence of Alpha and Delta lineages RNA in wastewater samples. This study provides a rapid, sensitive and cost-effective tool for detecting and classifying two worldwide dominant SC-2 variants. It also highlights the importance of a timely diagnostic response to the emergence of new SC-2 variants with significant consequences on global health. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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