32ULTRASOUND MEASUREMENTS OF BOVINE SOMATIC CLONES AT DAY 50 AND DAY 64 OF PREGNANCY
| Autor: | Fabienne Constant, Yvan Heyman, Michel Guillomot, D. LeBourhis, C. Juillien, Jean-Paul Renard, Pascale Chavatte-Palmer, P. Laigre |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Fetus
medicine.medical_specialty Pregnancy Offspring Theriogenology Embryo culture Embryo Reproductive technology Biology medicine.disease Andrology Endocrinology medicine.anatomical_structure Reproductive Medicine Lactation Immunology Genetics medicine Animal Science and Zoology Molecular Biology Developmental Biology Biotechnology |
| Zdroj: | Reproduction, Fertility and Development. 16:138 |
| ISSN: | 1031-3613 |
| DOI: | 10.1071/rdv16n1ab32 |
| Popis: | The overall efficiency of cloning has stayed low since this technique appeared. Most of the losses in the bovine species take place in early gestation between Day 35 and Day 70, when deficient placental development has been described. Late fetal losses are associated with excessive fetal growth and placental hydrops (Large Offspring Syndrome or LOS). One study measuring crown-rump length (CRL) by ultrasound (US) did not show any difference between clones and controls (Pace et al., 2002). The objective of this work was to compare fetal and placental measurements at Days 34, 50, and 64 for bovine clones and control pregnancies to determine whether these could be predictive of a further development of LOS. Twenty-five clone and 32 control (28 AI and 4 IVF) singleton Holstein pregnancies were used for US with a 5-MHz rectal probe. Clones originated from adult skin fibroblasts (three genotypes), used after serum starvation, as previously published (Vignon et al., 1998). CRL and head length (HL), orbital diameter (OD), vesicle width (VW), and length and width of the placentome closest to the fetus were measured at each stage. CRL were also recorded from six clone, six IVF and five AI, Day 34 and Day 60 fetuses recovered at slaughter. Maternal clinical parameters, US images with increased fetal fluids, excessive weight, and clinical features at surgical removal or birth were used to diagnose LOS. Data were analysed using multiple regression analysis with SAS software. There were no differences between IVF and AI fetal and placental measurements by ultrasound and the two groups were pooled as a single control group. LOS was diagnosed in 29% of the clones. CRL and HL were significantly smaller in clones at all stages (CRL: 10.2±3.8mm, n=5 v. 13.6±2.4mm, n=7, at retrieval at Day 34; 31.8±5.6mm v. 36.0±4.6 in controls at Day 50, by US, and 48.6±11.4 v. 63.3±4.9 at Day 64, by US, P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|