Nanotechnology-based combination therapy improves treatment response in cancer models
| Autor: | Prakash Rai, Daniel Neuman, Zhiming Mai, Sung K. Chang, Tayyaba Hasan |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
genetic structures
Combination therapy biology business.industry Panca medicine.medical_treatment Cancer Nanotechnology Photodynamic therapy medicine.disease biology.organism_classification eye diseases Vascular endothelial growth factor chemistry.chemical_compound chemistry Pancreatic cancer medicine Photosensitizer business Cytotoxicity |
| Zdroj: | SPIE Proceedings. |
| ISSN: | 0277-786X |
| DOI: | 10.1117/12.828402 |
| Popis: | Pancreatic cancer (PanCa) has a poor prognosis with a 5-year survival rate of only 5%. Photodynamic therapy (PDT) has shown promising results in treating PanCa. Mechanism-based combinations with PDT have enhanced treatment outcome. Agents tested with PDT include Avastin, an antibody against vascular endothelial growth factor (VEGF) which is approved for treating various cancers. Simultaneous delivery of drugs in nano-constructs could improve the treatment response of mechanism based combination therapies. Here, we investigate the effect of neutralizing VEGF using nanotechnology for the delivery of Avastin in combination with PDT. For this we used a construct called "nanocells" in which the photosensitizer was trapped inside polymer nanoparticles and these, with Avastin, were then encapsulated inside liposomes. In vitro, nanocells containing Avastin (NCA) significantly enhanced cytotoxicity in PanCa cells. NCA based PDT also significantly improved treatment response in mice that were orthotopically implanted with PanCa. Avastin delivered extracellularly in combination with PDT did not show any improvement. Here we propose a new paradigm for Avastin-based therapy by combining intracellular delivery of the antibody and PDT using nanotechnology for treating PanCa. |
| Databáze: | OpenAIRE |
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