| Autor: |
Lorenz, André, Lorenz, Melanie, Vothknecht, Ute, Niopek-Witz, Sandra, H. Neuhaus, Haferkamp, Ilka |
| Rok vydání: |
2015 |
| DOI: |
10.6084/m9.figshare.c.3630707_d7 |
| Popis: |
Heterologous expression and ATP transport analysis of N- terminally truncated AtAPC2. (A) SDS-PAGE of 5μg and (B) Western-blot and immunodetection of 0.5 μg of the inclusion bodies fraction from E. coli cells expressing the N-terminally truncated (lanes 1). To enable detection of the molecular mass reduction due to loss of the N-terminal extension the full-length protein was included in this analysis (lanes 2). The Western-blot was immuno-decorated with a monoclonal anti poly His IgG (Sigma, Taufkirchen, Germany). M, prestained molecular weight marker (Thermo Fisher Scientific). (C) Time dependent import of 50 μM [α32P]-ATP via N- terminally truncated AtAPC2 into ATP loaded (black rhombs), Pi loaded (gray circles) and non-loaded (non-filled rhombs) liposomes. (PDF 156 kb) |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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