Near-Field Imaging of Cell Membranes in Liquid Enabled by Active Scanning Probe Mechanical Resonance Control
| Autor: | Jung Hwa Kim, El-Hang Lee, Kyoung-Duck Park, Markus B. Raschke, Seung Gol Lee, Min Jung Jang, Beom-Hoan O, Se-Geun Park |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Materials science Nanotechnology 02 engineering and technology 021001 nanoscience & nanotechnology Near field imaging Surfaces Coatings and Films Electronic Optical and Magnetic Materials law.invention 03 medical and health sciences 030104 developmental biology General Energy Membrane Optical microscope Intracellular organelle law Mechanical resonance Near-field scanning optical microscope Physical and Theoretical Chemistry 0210 nano-technology Image resolution Nanoscopic scale |
| Zdroj: | The Journal of Physical Chemistry C. 120:21138-21144 |
| ISSN: | 1932-7455 1932-7447 |
| Popis: | Despite the power of far-field super-resolution microscopies for three-dimensional imaging of biomolecular structures and processes, its application is challenged in dense and crowded samples and for certain surface and membrane studies. Although near-field imaging with its ability to provide intrinsic subdiffraction limited spatial resolution at any optical modality, its application to biological systems has remained limited because of the difficulties of routine operation in liquid environments. Here we demonstrate stable and sensitive near-field scanning optical microscopy (NSOM) in a liquid based on a new mechanical resonance control and an optimization of the tip length, achieving a high quality factor (>2800) force sensing of the near-field probe. Through near-field imaging of the spatial distribution of epidermal growth factor receptors (EGFRs) on the membrane of A431 cancer cells as an example, we reveal nanoscale correlations between surface EGFR and intracellular organelle structures with ∼50 nm... |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|