Aquabacter cavernae sp. nov., a bacterium isolated from cave soil
| Autor: | Pei-Xiang Yang, Xing-Kui Zhou, Qi-Yan Cha, Ming-Liang Zhu, Qin Shichun, Yan-Qing Duan, Ti-Kun Zhang, Ming He Mo, Jin-Ling Duo |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine food.ingredient Stereochemistry Biology medicine.disease_cause 010603 evolutionary biology 01 natural sciences Microbiology 03 medical and health sciences chemistry.chemical_compound food medicine Xanthobacter Ecology Evolution Behavior and Systematics Aquabacter Phosphatidylglycerol Strain (chemistry) Phylogenetic tree General Medicine biology.organism_classification 16S ribosomal RNA 030104 developmental biology chemistry Xanthobacter tagetidis Bacteria |
| Zdroj: | International Journal of Systematic and Evolutionary Microbiology. 69:3716-3722 |
| ISSN: | 1466-5034 1466-5026 |
| DOI: | 10.1099/ijsem.0.003585 |
| Popis: | A Gram-stain-negative, rod-shaped, non-motile, aerobic, catalase-negative and oxidase-positive bacterium, designated strain Sn-9-2T, was isolated from a cave soil sample collected from Tiandong cave, Guizhou Province, south-west PR China. Growth occurred at 15–40 °C (optimum, 30 °C), at pH 5.0–9.0 (optimum, pH 7.0–8.0) and with 0–1 % NaCl (w/v). The predominant respiration quinone was ubiquinone-10 (Q-10). The major cellular fatty acids were summed feature 8 (C18 : 1ω7c or C18 : 1ω6c; 83.9 %) and C16 : 0 (5.8 %). The major polar lipids were phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidylcholine, phosphatidylglycerol, three unidentified phospholipids, two unidentified glycolipids, two unidentified polar lipids and one unidentified aminolipid. The DNA G+C content of strain Sn-9-2T was 67.5 mol%. Based on the results of 16S rRNA gene sequence analysis, the nearest phylogenetic neighbours of strain Sn-9-2T (MF958452) were identified as Aquabacter spiritensis (FR733686) DSM 9035T (97.5 %), Xanthobacter autorophicus (jgi.1053054) DSM 432T (97.2 %) and Xanthobacter tagetidis ATCC 700314T RCTF01000015 (96.9 %). The average nucleotide identity values were 78.0, 77.4 and 77.6 % and the digital DNA–DNA hybridization values were 21.8, 22.0 and 18.8 % between strain Sn-9-2T and A. spiritensis DSM 9035T, X. autotrophicus DSM 432T and X. tagetidis DSM 11105T, respectively. The DNA–DNA hybridization data indicated that strain Sn-9-2T represented a novel genomic species. On the basis of the results of phylogenetic analysis, chemotaxonomic data, physiological characteristics and DNA–DNA hybridization data, strain Sn-9-2T should represent a novel species of the genus Aquabacter , for which the name Aquabacter cavernae sp. nov. is proposed. The type strain is Sn-9-2T (=KCTC 62308T=CCTCC AB 2018270T). |
| Databáze: | OpenAIRE |
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