Insecticidal Compound from Himatanthus drasticus Latex against Cowpea Infestation by Callosobruchus maculatus (Coleoptera: Chrysomelidae)
| Autor: | Alison B. da Silva, José Francisco de Carvalho Gonçalves, Ayrles F.B. Silva, Márcio V. Ramos, Paulo Riceli Vasconcelos Ribeiro, Otília Deusdênia L. Pessoa, Brandon Ferraz E Sousa, Cleverson D.T. Freitas, Francimauro S. Morais, Emanuel Alves Bezerra, Diego P. Souza, Kirley Marques Canuto |
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| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
Larva Chloroform biology Starch Weevil 010401 analytical chemistry food and beverages General Chemistry biology.organism_classification medicine.disease_cause 01 natural sciences 0104 chemical sciences Callosobruchus maculatus Vigna chemistry.chemical_compound Horticulture chemistry Infestation medicine Postharvest General Agricultural and Biological Sciences 010606 plant biology & botany |
| Zdroj: | Journal of Agricultural and Food Chemistry. 69:5049-5058 |
| ISSN: | 1520-5118 0021-8561 |
| Popis: | Vigna unguiculata is an important source of proteins and energy for humans and animals. However, postharvest losses caused by Callosobruchus maculatus can reach from 20 to 100% of stored seeds. In this study, the insecticide potential of compounds extracted from Himatanthus drasticus latex was assessed. The latex was extracted with ethanol (70%) and then partitioned through sequential use of hexane and chloroform. These fractions were investigated by chromatography to determine their chemical composition. Plumieride, identified in a hydroalcoholic subfraction, was tested for insecticidal activity against C. maculatus. The ethanolic fraction (LC50 = 0.109; LC90 = 0.106%) and the plumieride (LC50 = 0.166; LC90 = 0.167%) were lethal to larvae. Plumieride (0.25%) delayed larval development, and mortality reached 100%. Its inhibitory action on intestinal α-amylase from larvae was higher (89.12%) than that of acarbose (63.82%). Plumieride (0.1%) inhibited the enzyme α-amylase in vivo in the larval intestine. This result was confirmed by a zymogram test performed by SDS-PAGE when the enzyme electrophoresed on gel copolymerized with starch. When spread on seeds, the hydroalcoholic fraction (1.0%) reduced infestation. The loss of seed mass was 5.26% compared to the control (44.97%). The results confirm the effect of latex compounds in protecting stored seeds against weevil infestation. |
| Databáze: | OpenAIRE |
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