Detection of Reactive Oxygen and Nitrogen Species (ROS/RNS) During Hypersensitive Cell Death
| Autor: | Laura Carmen Terrón-Camero, Eliana Molina-Moya, Luisa M. Sandalio, María Sanz-Fernández, María C. Romero-Puertas |
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| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine chemistry.chemical_classification Hypersensitive response Reactive oxygen species Cell signaling Programmed cell death biology food and beverages biology.organism_classification 01 natural sciences Cell biology Nitric oxide 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology chemistry In vivo Arabidopsis Peroxynitrite 010606 plant biology & botany |
| Zdroj: | Methods in Molecular Biology ISBN: 9781493976676 |
| DOI: | 10.1007/978-1-4939-7668-3_9 |
| Popis: | Reactive oxygen and nitrogen species (ROS/RNS) are signaling molecules involved in a plethora of physiological processes in plants. Especially, ROS and nitric oxide (NO) are key players that are required for programmed cell death (PCD). The PCD associated with the hypersensitive response (HR) has been well characterized and the role of H2O2 and NO as key signaling molecules inducing HR has been established. Localization of ROS and NO production in plant tissues in response to pathogens can be imaged by confocal laser microscopy by using specific fluorescent probes. Deciphering the time and spatial regulation of ROS and NO is very important to establish the cellular response of plants to adverse conditions. This chapter is mainly focused on the imaging of ROS and RNS accumulation in vivo in plant tissues undergoing PCD. |
| Databáze: | OpenAIRE |
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