Antigenic Epitopes on Human P-glycoprotein Recognized by Autoimmune Hepatitis Autoantibody as a Case Study
| Autor: | Takaharu Mizutani, Masatoshi Masuda |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
chemistry.chemical_classification
biology business.industry Health Toxicology and Mutagenesis Autoantibody Peptide Autoimmune hepatitis Toxicology medicine.disease_cause medicine.disease Virology Epitope Autoimmunity Transmembrane domain chemistry Antigen Immunology biology.protein Medicine Antibody business |
| Zdroj: | Journal of Health Science. 53:282-290 |
| ISSN: | 1347-5207 1344-9702 |
| DOI: | 10.1248/jhs.53.282 |
| Popis: | Multiple drug resistant protein 1 (MDR1), P-glycoprotein, plays a role in the blood-brain barrier, preventing drug distribution into the brain, and in cancer chemotherapy. MDR1 is composed of two repeated fragments and there are six transmembrane domain (TMD) on the N-terminal of each repeat and a nucleotide-binding domain (NBD) on the C-terminal. We reported that sera from autoimmune hepatitis patients well reacted with MDR1 by enzyme-linked immuno-sorbent assay (ELISA) [Shinoda et al., (2004) Autoimmunity, 37, 473–480]. In this study, to determine antigenic sites, peptides on the extra-cellular loop (ECL), TMD and NBD of MDR1 were applied to ELISA with sera from 4 autoimmune hepatitis (AIH) patients and 4 normal individuals. The results showed that serum from Patient 3 reacted well with peptide 314–328 an dw eakly with peptide 957–971. Meanwhile, serum from Patient 4 reacted well with peptide 850–857 and weakly with peptide 741–755 and 957–971. All the five peptides reacted with sera from Patients 3 and 4 were located on ECL. Normal sera did not react with those peptides and the reactions of sera from Patients 1 and 2 were marginal. Sera from 4 patients and normal individuals did not react with peptides of TMD and NBD. These results suggest that some ECL on MDR1 play a role of antigenic determinants, and TMD and NBD do not. Personal specificity and diversity of antibodies from the AIH patients (such as Patients 3 and 4) against antigenic determinant were found. |
| Databáze: | OpenAIRE |
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