Rosmarinic acid attenuated SIN-1-induced cytotoxicity in HepG2 cells through the HO-1 induction and radical scavenging activity
| Autor: | Dae Seong Choi, Hee Sun Yang, Myung Woo Byun, Wang Geun Kim, Chang Hyun Jin, Il Yun Jeong |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
MAPK/ERK pathway
chemistry.chemical_classification Reactive oxygen species Phosphoinositide 3-kinase biology Chemistry Rosmarinic acid Chromosomal translocation Applied Microbiology and Biotechnology Molecular biology chemistry.chemical_compound Biochemistry biology.protein Cytotoxicity Heme PI3K/AKT/mTOR pathway Food Science Biotechnology |
| Zdroj: | Food Science and Biotechnology. 22:549-556 |
| ISSN: | 2092-6456 1226-7708 |
| DOI: | 10.1007/s10068-013-0113-x |
| Popis: | Rosmarinic acid (RA), a food-derived polyphenolic compound, has been reported to possess anti-oxidant, antiallergic, and anti-inflammatory activities. In this study, we investigated whether RA has cytoprotective activity in SIN-1 induced HepG2 cells and how to achieve it. The results show that RA attenuates SIN-1 induced cytotoxicity in HepG2 cells. RA significantly reduces the intracellular reactive oxygen species (ROS) generated from SIN-1 and induced heme oxygenase-1 (HO-1) expression. RA-induced HO-1 expression was attenuated by a pretreatment with specific inhibitors for JNK, ERK, and phosphoinositide 3-kinase (PI3K). The addition of N-acetyl-cysteine (NAC) also reduced HO-1 induction in RA-induced cells. Furthermore, RA induced the translocation of NF-E2-related factor 2 (Nrf2). Taken together, we conclude that RA attenuates cytotoxicity in SIN-1-induced HepG2 cells by direct radical scavenging activity and HO-1 induction through a ROS/PI3K/MAPKs/Nrf2 pathway. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink