Isolation and Characterization of Brassicasterol from N-Hexane Fraction of Pometia pinnata and its Toxicity Test

Autor:	Mai Efdi, Yoan De Nanda Herru Adlis Santoni
Rok vydání:	2021
Předmět:	Hexane chemistry.chemical_compound Chromatography biology Chemistry Toxicity Fraction (chemistry) Pometia pinnata Brassicasterol Isolation (microbiology) biology.organism_classification
Zdroj:	International Journal of Current Microbiology and Applied Sciences. 10:207-215
ISSN:	2319-7706 2319-7692
DOI:	10.20546/ijcmas.2021.1009.024
Popis:	Pometia pinnata leaves were extracted and fractionated using n-hexane, dichloromethane, ethyl acetate, and methanol. The four fractions obtained were screened for cytotoxic testing using the Brine shrimp lethality test (BSLT) method, n-hexane fraction has the highest LC50 419,855 mg/L.The n-hexane fraction was continued for the isolation stage and a secondary metabolite compound was obtained, namely brassicasterol. The structure of this secondary metabolites was determined using spectroscopic methods (UV-Vis, FTIR, and NMR).
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::ab9246188d284a5d115a46b308fcf86a https://doi.org/10.20546/ijcmas.2021.1009.024 Zobrazit plný text záznamu