Brief communication. Tetraploid nature of Sorghum bicolor (L.) Moench

Autor:	Rod A. Wing, M. N. Islam-Faridi, H. J. Price, Michael S. Zwick, G. E. Hart, D. G. Czeschin, M. I. Gomez, David M. Stelly
Rok vydání:	1998
Předmět:	Genetics Bacterial artificial chromosome Chromosomes E food and beverages Sorghum bicolor Biology Sorghum biology.organism_classification Genome Plant chromosomes Centromere Molecular Biology Genetics (clinical) Biotechnology Subgenomic mRNA
Zdroj:	Journal of Heredity. 89:188-190
ISSN:	1465-7333 0022-1503
DOI:	10.1093/jhered/89.2.188
Popis:	The advent of bacterial artificial chromosomes (BACs) and development of high quality methods for fluorescent in situ hybridization (FISH) to plant chromosomes offer revolutionary tools for plant genome analysis. We discovered a 45 kb sorghum BAC (22B2) that differentially hybridizes to centromere regions of 10 of the 20 chromosomes of sorghum (Sorghum bicolor). Moreover, hybridization of this BAC to plants trisomic (2n = 20 + 1) for the five available trisomes identified their respective subgenomic affiliations. Plants trisomic for chromosomes E, H, and I displayed 11 signals, indicating that these chromosomes are in the subgenome marked by the BAC-FISH signal, whereas plants trisomic for chromosomes D and G had only 10 signals and therefoe belong to the subgenome not displaying the FISH signals. The results provide strong evidence that sorghum is at least of tetraploid origin, and that there are two subgenomes of five chromosomes each in the S. bicolor genome.
Databáze:	OpenAIRE
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